scramble control  (Addgene inc)

Journal: Cell Death Discovery

Article Title: SPINK2 silencing suppresses leukemic proliferation and restores myeloid commitment via MECOM downregulation in acute myeloid leukaemia

doi: 10.1038/s41420-026-02988-1

Figure Lengend Snippet: A Barplot showing the expression of SPINK2 measured by qPCR in a panel of AML cell lines. n = 6 for each cell line. B Bowtie plot representing SPINK2 mRNA quantification by q-PCR following SPINK2 shRNA silencing and normalized using GAPDH ( n = 6). C Lineplot displaying cell viability upon SPINK2 knock-down determined by counting cells every 24 h for four consecutive days post shRNA induction. The results are indicative of 3 independent experiments ( n = 6). D Two-dimensional flow cytometric dot plot showing BrdU incorporation 72 hours post doxycycline induction. The three gates indicate the G0/G1, S and G2/M phases of the cell cycle. The barplot on the righthand side represent an average of three independent experiments ( n = 6). E Bowtie plot showing the percentages of Annexin V + apoptotic/necrotic cells. F Representative two-dimensional flow cytometric contour plots showing the expression levels of CD14, CD64, CD35 and CD300E in FUJIOKA cells that have undergone SPINK2 silencing 96 hours post doxycycline induction ( n = 6). The statistical analysis reported in this figure have been performed using student’s t test (*** p < 0.001, * p < 0.05).

Article Snippet: A scrambled control shRNA plasmid (shCtr), Tet-pLKO-puro-Scrambled (Addgene plasmid #47541), was used as a non-targeting control [ ].

Techniques: Expressing, shRNA, Knockdown, BrdU Incorporation Assay

Journal: The Journal of Biological Chemistry

Article Title: Cytosolic nucleic acid sensing triggers type I interferon activation via the Hippo kinase LATS1

doi: 10.1016/j.jbc.2026.111204

Figure Lengend Snippet: LATS1 operates at the level of TBK1. A , immunoblot analysis of LATS1 expression in HEK 293T cells treated with control siRNA or siRNAs targeting LATS1 for 24 h. B , IFN-β luciferase reporter assay in HEK 293T cells treated with control siRNA or siRNAs targeting LATS1 as in A , followed by transfection of plasmids encoding RIG-I, MAVS, STING, TBK1, and IRF3. C , co-immunoprecipitation and immunoblot of FLAG-LATS1 and HA-TBK1 co-transfected in HEK 293T cells. D and E , immunoblot analysis of TBK1-LATS1 interactions in MEF cells transfected with pI:C ( D ) or ISD ( E ) (2.5 μg/ml each; 2 h). Statistical significance was determined using student’s t test (∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).

Article Snippet: For small interfering RNA (siRNA) studies, HEK 293T cells were transfected with 60 pmol of control scrambled siRNA or 3 different siRNA targeting human LATS1 (20 pmol each) (Bioneer) using Lipofectamine 2000 (Invitrogen).

Techniques: Western Blot, Expressing, Control, Luciferase, Reporter Assay, Transfection, Immunoprecipitation